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Jonathan Tyson is a Propel Postdoctoral Research Fellow currently working jointly between the labs of Carolyn Bertozzi (Department of Chemistry), Karl Deisseroth (Department of Bioengineering) and Zhenan Bao (Department of Chemical Engineering) at Stanford University. Jonathan began his academic career as a McNair scholar at Rider University, where he studied biochemistry and mathematics, and performed research with Dr. Danielle Jacobs towards the total asymmetric synthesis of gamma butyrolactone-containing natural products. Upon his graduation, Jonathan was recognized as the most outstanding Organic Chemistry Undergraduate by Rider University and the Trenton local ACS. Following graduation, Jonathan began his Ph.D. in chemistry at Yale University, where he worked in the lab of Alanna Schepartz on the design and synthesis of novel photoactive chemical tools for live cell super-resolution microscopy. While at Yale, Jonathan also worked for three years in the Center for Teaching and Learning and worked closely with the Office for Graduate Diversity. In January 2020, the Schepartz lab moved from Yale to UC Berkeley, where Jonathan completed the final stages of his Ph.D., and was a Graduate Diversity Fellow and active member of Berkeley’s Black Graduate Engineering and Science Students (BGESS). As a postdoc, Jonathan is working to enable genetically targeted chemical assembly of functional bioelectronics in vivo. 

Peaking Inside Cells At Super-resolution With Rationally Designed Chemical Tools

True molecular understanding of biology requires the characterization of cellular structures with high spatial resolution within live cells. Super-resolution microscopy methods like single molecule localization microscopy enable high resolution imaging, but the application of this technique to efficient live cell imaging has been limited by a shortage of available fluorophores. New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.

 

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This event is open to members of the Reed Community and their invited guests.  Chemistry Students can access the Zoom link via the Chemistry Majors Moodle. If you do not have access the Chemistry Majors Moodle please email johnstonk@reed.edu for the Zoom link.