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Biology Seminar: Derek Applewhite, "The Spatial and Temporal Regulation of Non-muscle myosin II Contractility"

This Friday's Biology Department Seminar speaker is:

Derek Applewhite, Ph.D
Department of Biology, Reed College

 “The Spatial and Temporal Regulation of Non-muscle myosin II Contractility”

Friday, September 14, 2018
4:10 pm Seminar begins
Biology 19

Student Lunch: There is no student lunch this week.

Abstract: Non-muscle Myosin II (NMII) generated contractility is a fundamental cellular process that occurs during cell migration and division, however it is particularly important to morphogenesis, or the cell shape change that occurs during development. NMII forms bi-polar anti-parallel filaments upon activation, binding to actin filaments to generate force. While many of the kinetic and biophysical properties of NMII are well known, the molecular cues dictating when and where it is activated are far less well understood. What is also lacking is a complete list of the molecules involved in the regulation filament dynamics and contractility. This research aims to dissect the recruitment and activation of NMII through the analysis of a novel, NMII regulatory molecule RN-tre. With RN-tre as an example, we will identify additional regulators of NMII contractility during this critical morphogenic process, apical constriction, using complementary experimental and computational methodologies. Drosophila tissue culture cells have been used in multiple studies that have made significant contributions to our knowledge of the cytoskeleton. Their sensitivity to RNAi, allowing for effective depletion of one or more proteins, and their confined geometry make them ideal for high-resolution imaging techniques such as total internal reflection microscopy (TIRF). Furthermore, this we will take advantage of testing computationally-predicted NMII regulators using a cell-based assay of apical constriction which will allow important questions to be asked about this developmental process, while side-stepping the complications of imaging in an embryo.

Additional Biology Department seminars can be found on-line, including links to some speakers' home pages at:

Friday, September 14, 2018 at 4:10am to 5:00pm

Biology, Biology 19
3203 Southeast Woodstock Boulevard, Portland, Oregon 97202-8199

Event Type



Faculty, Students, Alumni, Open to the Public, Parents & Family, Staff, Prospective Students


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